Adaptive Optics Microscope Software

Adaptive optics is a technique used to correct for imaging aberrations. It is most famously known in astronomy where ground-based telescopes correct for how the atmosphere tends to bend and scatter starlight. In the case of biological microscopy, the technique can be adapted to correct for the biological aberrations. Here many images are initially taken to characterize the aberrations that each light path through the sample experiences. A spatial light modulator is then used to steer the parts of the light beam back into sharp focus. To develop the instrument control program to carry out this technique, the researchers worked with Dr. Daniel Milkie of Sciotex to build the LabVIEW program that would run the 2-photon microscope, analyze the images, and optimize the adaptive optics to correct the aberrations. This success of this work was recently published in Nature Methods. Nature Methods Article Abstract